In vitro Cultivation of the Entomogenous Nematode Filipjevimermis leipsandra
Abstract
The mermithid nematode, Filipjevimermis leipsandra, was successfully cultivated to the preadult stage in Schneider's Drosophila medium supplemented with 20% fetal bovine serum. Upon transfer to a solid substrate the preadults continued to develop into ovipositing adult females. Four molts were observed. The first molt occurred in the egg. The second occurred after 6-8 days in culture during which the very thin cuticle was shed completely. The third molt occurred after 18-20 days in culture; the cuticle was retained by the third-stage nematode. This stage was considered comparable to the preadult stage that emerges from host larva, Diabrotica spp. The fourth molt occurred within 12 days after the preadult was transferred from the liquid medium to a solid substrate. Adult females began ovipositing viable eggs 1-3 days after the final molt. Key words: Diabrotica balteata, banded cucumber beetle, mermithid.Downloads
Published
Issue
Section
License
Copyright and Permissions
All material published by the Society of Nematologists (SON), except for papers prepared by United States and Canadian government employees, is copyrighted and protected under the U.S. copyright law. Under the Copyright Act of 1976, the term of copyright for materials registered by an organization is 75 years from the date first published. Before publishing any manuscript, SON requires that authors transfer full and complete ownership of any copyright to SON by signing a JON Page Charge/Copyright Form (.pdf). SON then registers the copyright. Subsequent use of published materials requires written permission from the SON and may be obtained by contacting the current Editor-in-Chief and state where and how the material will be used.
The author warrants that the article is an original work not published elsewhere in whole or in part, except in abstract form, and that the author has full power to make this grant. If portions of the article have been published previously, then the author warrants that permission has been obtained from the copyright holder and the author will submit a copy of the permission release with this copyright transfer form.
SON shall claim no proprietary right other than copyright. Authors and coauthors retain the right to revise, adapt, modify, or otherwise use all or part of the article in future works of the author(s), such as press releases, lectures, and reviews, provided that all such use is for the personal noncommercial benefit of the author(s). All patent rights are retained by the author(s).
