Cryofracturing for Scanning Electron Microscope Observations of Internal Structures of Nematodes
Abstract
Nematodes were prepared for scanning electron microscopy by cryofracturing in ethanol and then by critical-point drying in carbon dioxide. Cross sections of Caenorhabditis briggsae and Xiphinema americanum showed the arrangement of the intestine, ovaries, muscle cells, and some layers of the cuticle. The technique is complementary to transmission electron microscopy and facilitates the interpretation of results from thin sections. Key Words: Caenorhabditis briggsae, Xiphinema americanum.Downloads
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