Toxicity of a novel formulation of Bigshot Maxim against Aedes aegypti

Abstract

A series of laboratory evaluations were conducted to assess the ADULTICIDAL efficacy of a non-commercial, experimental formulation of BIGSHOT Maxim (PreVasive USA, LLC) against Aedes aegypti Linn. mosquitoes. The formulation is a glycerine-based polymer microencapsulated cedar, thyme, cinnamon, peppermint, and lemongrass organic oils (18.8% active ingredient by weight). Efficacy of the microencapsulated formulation was evaluated via CDC bottle bioassay and wind tunnel testing and compared against the original BIGSHOT Maxim formulation. Three bottle bioassay trials were conducted evaluating concentrations of both test materials at 13.3, 6.6, 4.4, and 3.3 µg/cm2 of active ingredient (A.I.), respectively. Four premade solutions were provided for the wind tunnel testing. These were prepared by mixing 4.0, 7.39, 14.1, and 29.57 mL of an 80 g/L of pyrethrum and 480 g/L of PBO solution into the novel BIGSHOT Maxim formulation to make 100 mL total volume. Bottle bioassay results for the microencapsulated formula ranged between 27.3-93.0% yielding 1h mortality greater than 80% at concentrations above 6.6 µg/cm2 demonstrating strong efficacy but failed to exceed the original formulation. Overall wind tunnel mortality at 24h was low, ranging between 10.0-22.0%.

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