In Vitro Propagation of Ipomoea pes-caprae (Railroad-Vine)

Authors

  • Michael E. Kane
  • Kimon T. Bird
  • Tammera M. Lee

Keywords:

Dune plants, micropropagation, cytokinins, coastal revegetation

Abstract

A plant micropropagation protocol for in vitro establishment, plant multiplication, rooting, and ex vitro acclimatization of the dune species Ipomoea pes-caprae (Railroad-vine) was developed. Supplementation of agar-solidified Murashige and Skoog medium (MS) with either the cytokinin 2- isopentenyladenine (2iP) or zeatin significantly promoted shoot regeneration from nodal segments. Maximum shoot regeneration occurred from nodal segments cultured on agar-solidified MS supplemented with 5 mg/liter 2iP. Medium supplementation with either 2iP or zeatin did not promote axillary branching of the shoots produced. Microcuttings were effectively rooted (95%) in vitro when cultured on agar-solidified full-strength MS supplemented with 1.0 mg/liter indolebutyric acid (lBA). Transplant survival of rooted I. pes-caprae microcuttings was 100%. Approximately 446,000 I. pes-caprae plants can be produced per year from a single nodal segment using this procedure


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Published

1993-04-10

Issue

Vol. 9 No. 2 (1993): Journal of Coastal Research

Section

Articles

License

Authorization for personal use is granted by the Coastal Education and Research Foundation [CERF]. By submitting a manuscript, the authors agree that the copyright for their article is transferred to the publisher if the article is accepted for publication. Assignment of copyright is not required, however, from authors who work for organizations which do not permit such assignment, The copyright covers the exclusive rights to reprduce and distribute the article, inclduing reprints, photographic reproductions, microform, or any other reproductions of a similar nature, and translations.