Abstract
We have conducted several interploid crosses between fertile somatic hybrids such as 'Hamlin' + 'Femminello', 'Key' lime + 'Valencia', 'Valencia' + 'Femminello', 'Milam' + 'Femminello' and 4X 'Femminello' and various diploid acidfruit cultivars to generate seedless triploids with horticulturally acceptable fruit and improved cold-hardiness (inherited from sweet orange), suitable for production in sub-tropical regions. Embryo rescue was utilized to recover triploid progeny about three months after pollination. Embryos in different developmental stages were grown on Murashige and Tucker medium supplemented with 500 mg/L malt extract. A total of fifty three triploids and twenty two tetraploids were recovered from different cross combinations.
