Vol. 125 (2012): Proceedings of the Florida State Horticultural Society
Handling & Processing

Detection of 16S rDNA of CandidatusLiberibacter asiaticus in Orange Juice by Quantitative Real-time PCR

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  • Jinhe Bai,
  • Elizabeth Baldwin,
  • Hui-Ling Liao,
  • Igor Kostenyuk,
  • Jacqueline Burns,
  • Mike Irey
Jinhe Bai
USDA, ARS, USHRL, 2001 S. Rock Road, Ft. Pierce, FL 34945
Elizabeth Baldwin
USDA, ARS, USHRL, 2001 S. Rock Road, Ft. Pierce, FL 34945
Hui-Ling Liao
University of Florida, IFAS, Citrus Research and Education Center, Lake Alfred, FL 33850
Igor Kostenyuk
University of Florida, IFAS, Citrus Research and Education Center, Lake Alfred, FL 33850
Jacqueline Burns
University of Florida, IFAS, Citrus Research and Education Center, Lake Alfred, FL 33850
Mike Irey
United States Sugar Corporation, 111 Ponce de Leon Avenue, Clewiston, FL 33440

Published 2012-12-01

Keywords

  • orange juice,
  • Huanglongbing,
  • amplification inhibitor,
  • pectin,
  • pH

Abstract

Orange juice processed from Huanglongbing (HLB) affected fruit is often associated with bitter taste and/or off-flavor. HLB disease in Florida is associated with CandidatusLiberibacter asiaticus (CLas), a phloem limited bacterium. The current standard to confirm CLas for citrus trees is to take samples from midribs of leaves, which are rich in phloem tissues, and use quantitative real-time polymerase chain reaction (qPCR) test to detect 16S rDNA gene of CLas. It is extremely difficult to detect CLas in orange juice because of the low CLas population, high sugar and pectin concentration, low pH and possible existence of an inhibitor to DNA amplification. The objective of this research was to improve extraction of DNA from orange juice, and detection of CLas by qPCR.

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