A comparative analysis of resistance testing methods in Aedes albopictus from St. John’s County, Florida

Authors

  • Christy Marie Waits Navy Entomology Center of Excellence, CMAVE Detachment, Gainesville, FL, USA
  • Ali Fulcher Anastasia Mosquito Control District, St. Augustine, FL, USA;
  • Jessica Eason Louton USDA/ARS/Center for Medical, Agricultural, and Veterinary Entomology, Mosquito & Fly Research Unit, Gainesville, FL, USA
  • Alec G. Richardson Navy Entomology Center of Excellence, Testing & Evaluation Department, NASJAX, Jacksonville, FL, USA
  • James J. Becnel USDA/ARS/Center for Medical, Agricultural, and Veterinary Entomology, Mosquito & Fly Research Unit, Gainesville, FL, USA
  • Rui-de Xue Anastasia Mosquito Control District, , St. Augustine, FL, USA
  • Alden Strother Estep Navy Entomology Center of Excellence, CMAVE Detachment, Gainesville, FL, USA http://orcid.org/0000-0002-8281-3229

Keywords:

Asian tiger mosquito, susceptibility, permethrin, bifenthrin, malathion,

Abstract

Abstract

Aedes albopictus Skuse (Diptera: Culicidae) was tested for resistance to permethrin, bifenthrin, and malathion using Centers for Disease Control and Prevention (CDC) bottle bioassays and topical toxicology assays on adults and larval bioassays. Eggs were collected from 3 locations across St. Johns County, Florida, raised to the F3 generation and compared with an insecticide susceptible laboratory strain. Results from CDC bottle bioassays with permethrin indicate no significant differences between the 3 wild-type strains and the laboratory strain but suggest the possibility of resistance in 1 strain. Bottle bioassay results for malathion were inconclusive. Topical toxicological results for adults and bioassays for larvae showed a significant difference in permethrin resistance between the control strain and 1 of the wild-type strains. Results from this project indicate that insecticide susceptibility testing should be a regular part of mosquito surveillance programs. Upon detection of resistance, detailed dose response bioassays should be performed to quantify the resistance and mechanisms in local vector populations.

 

Resumen

Aedes albopictus Skuse (Diptera: Culicidae) fue sometida a pruebas de resistencia a permetrina, bifentrina y malatión utilizando bioensayos con botellas del CDC (Centro de Control de Enfermedades y Prevención) y ensayos de toxicología en larvas y adultos. Se recolectaron huevos de tres localidades del condado de St. Johns, Florida, los cuales fueron criados hasta la generación F3 y fueron probardos contra una cepa de laboratorio susceptible al. Los resultados de los bioensayos con botellas de CDC no indican diferencias significativas entre las tres cepas de tipo salvaje y la cepa de laboratorio, pero sugieren la posibilidad de resistencia a la permetrina en una cepa. Los resultados del bioensayo de botella para el malatión fueron inconclusivos. Los resultados toxicológicos para adultos y larvas mostraron diferencias significativas entre la cepa control y una de las cepas silvestres para la resistencia a la permetrina. Los resultados de este proyecto indican que las pruebas de susceptibilidad a los insecticidas deben formar parte de los programas de vigilancia de los mosquitos. En caso de detectar resistencia, deben realizarse bioensayos detallados de dosis-respuesta para cuantificar la resistencia y los mecanismos en las poblaciones locales de vectores.

 

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Author Biographies

Christy Marie Waits, Navy Entomology Center of Excellence, CMAVE Detachment, Gainesville, FL, USA

Bioscience Technologist

NECE, CMAVE Detachment

Ali Fulcher, Anastasia Mosquito Control District, St. Augustine, FL, USA;

Biologist

Anastasia Mosquito Control District

Jessica Eason Louton, USDA/ARS/Center for Medical, Agricultural, and Veterinary Entomology, Mosquito & Fly Research Unit, Gainesville, FL, USA

Biological Science Technician,

Mosquito & Fly Research Unit,

Center for Medical, Agricultural, and Veterinary Entomology

Alec G. Richardson, Navy Entomology Center of Excellence, Testing & Evaluation Department, NASJAX, Jacksonville, FL, USA

Biostatistician, NECE, Testing & Evaluation Department

James J. Becnel, USDA/ARS/Center for Medical, Agricultural, and Veterinary Entomology, Mosquito & Fly Research Unit, Gainesville, FL, USA

Research Entomologist

Mosquito & Fly Research Unit,

Center for Medical, Agricultural, and Veterinary Entomology

Rui-de Xue, Anastasia Mosquito Control District, , St. Augustine, FL, USA

Director,

Anastasia Mosquito Control District

Alden Strother Estep, Navy Entomology Center of Excellence, CMAVE Detachment, Gainesville, FL, USA

Entomologist

NECE, CMAVE Detachment

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Published

2017-10-10

Issue

Section

Research Papers