A comparative analysis of resistance testing methods in Aedes albopictus from St. John’s County, Florida
Keywords:
Asian tiger mosquito, susceptibility, permethrin, bifenthrin, malathion,Abstract
Abstract
Aedes albopictus Skuse (Diptera: Culicidae) was tested for resistance to permethrin, bifenthrin, and malathion using Centers for Disease Control and Prevention (CDC) bottle bioassays and topical toxicology assays on adults and larval bioassays. Eggs were collected from 3 locations across St. Johns County, Florida, raised to the F3 generation and compared with an insecticide susceptible laboratory strain. Results from CDC bottle bioassays with permethrin indicate no significant differences between the 3 wild-type strains and the laboratory strain but suggest the possibility of resistance in 1 strain. Bottle bioassay results for malathion were inconclusive. Topical toxicological results for adults and bioassays for larvae showed a significant difference in permethrin resistance between the control strain and 1 of the wild-type strains. Results from this project indicate that insecticide susceptibility testing should be a regular part of mosquito surveillance programs. Upon detection of resistance, detailed dose response bioassays should be performed to quantify the resistance and mechanisms in local vector populations.
Resumen
Aedes albopictus Skuse (Diptera: Culicidae) fue sometida a pruebas de resistencia a permetrina, bifentrina y malatión utilizando bioensayos con botellas del CDC (Centro de Control de Enfermedades y Prevención) y ensayos de toxicología en larvas y adultos. Se recolectaron huevos de tres localidades del condado de St. Johns, Florida, los cuales fueron criados hasta la generación F3 y fueron probardos contra una cepa de laboratorio susceptible al. Los resultados de los bioensayos con botellas de CDC no indican diferencias significativas entre las tres cepas de tipo salvaje y la cepa de laboratorio, pero sugieren la posibilidad de resistencia a la permetrina en una cepa. Los resultados del bioensayo de botella para el malatión fueron inconclusivos. Los resultados toxicológicos para adultos y larvas mostraron diferencias significativas entre la cepa control y una de las cepas silvestres para la resistencia a la permetrina. Los resultados de este proyecto indican que las pruebas de susceptibilidad a los insecticidas deben formar parte de los programas de vigilancia de los mosquitos. En caso de detectar resistencia, deben realizarse bioensayos detallados de dosis-respuesta para cuantificar la resistencia y los mecanismos en las poblaciones locales de vectores.
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