Photosensitizing effects of hematoporphyrin dihydrochloride against the flesh fly Parasarcophaga argyrostoma (Diptera: Sarcophagidae)

Authors

  • Salaheldin A. Abdelsalam Department of Biological Sciences, Faculty of Science, King Faisal University, Al-Ahsa 31982, P.O. Box 400, Saudi Arabia Zoology Department, Faculty of Science, Assiut University, Egypt
  • Ahmed M. Korayem Zoology Department, Faculty of Science, Assiut University, Egypt
  • Elsayed A. M. Elsherbini Department of Measurements, Photochemistry and Agriculture Applications, National Institute of Laser Enhanced Science, Cairo University, Egypt
  • Abdel-Aal A. Abdel-Aal Zoology Department, Faculty of Science, Assiut University, Egypt
  • Doaa S. Mohamed Zoology Department, Faculty of Science, Assiut University, Egypt

Keywords:

acetylcholine esterase, antioxidant enzymes, midgut, hematoporphyrin dihydrochloride, Parasarcophaga argyrostoma, photosensitizer

Abstract

There is an urgent need to use environmentally safe pesticides, which do not sneak into human food or animals and then back at him and damage the environment. The photosensitizers, such like hematoporphyrin dihydrochloride (HpD), a nontoxic chemical has an ability to harm the target pest when mixed with suitable baits. When exposed to light, the ingested HpD triggers the destruction of the midgut and inhibits the defensive enzymes, viz acetylcholine esterase (AchE), superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST). In the current study, HpD was investigated as photoinsecticide against 3rd larval instar and adults of a myiatic fly, Parasarcophaga argyrostoma (Robineau-Desvoidy) (Sarcophagidae: Diptera). Third instar larvae and adults were fed baits containing 5×10−2, 5× 10−3, 5× 10−4, 5× 10−5, and 5× 10−6 ppm of HpD. Mortality ratios were recorded at different post treatment sunlight exposure periods. The biochemical study of the impact of one median concentration, 5× 10−4 ppm, proved its ability to cause damage that may be beyond repair for some defensive enzymes. In addition, fatal histopathological changes were detected in the midgut epithelium. The study showed that, HpD could be an excellent pesticide against the larvae and adults of the myiatic flies.

 

Existe una necesidad urgente de utilizar pesticidas ecológicamente seguros, que no se filtren dentro alimentos para humanos o animales. Los fotosensibilizadores, como dihidrocloruro de hematoporfirina (HPD), tienen la capacidad de dañar a la plaga de enfoque de estudio cuando se mezcla con los cebos adecuados. Cuando se expone a la luz, el HpD ingerido provoca la destrucción del intestino medio e inhibe las enzimas defensivas, como las enzimas antioxidantes y de desintoxicación. En el presente estudio, se investigó HpD como un photoinsecticida contra el tercer estadio larval y adultos de una mosca miática, Parasarcophaga argyrostoma (Robineau-Desvoidy) (Sarcophagidae: Diptera). Para investigar los efectos de fotodinámicos de HpD, larvas del tercer estadio y adultos fueron tratados con diluciones en serie de 5 × 10−2, 5 × 10−3, 5 × 10−4, 5 × 10−5 y 5 × 10−6 ppm. Se registró la tasa de mortalidad en diferentes períodos de exposición a la luz de sol pos-tratamiento. El estudio bioquímico del impacto de una concentración mediana, 5 × 10−4 ppm, demostró su capacidad de causar daños que pueden estar más allá de la reparación de algunas enzimas defensivas. Además, se detectaron cambios histopatológicos fatales en el epitelio del intestino medio. El estudio mostró que, HpD podría ser una excelente pesticida contra larvas y adultos de moscas miática.

 

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Issue

Vol. 97, No. 4 (December 2014)

Section

Research Papers

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