Multiple gene markers to understand genetic diversity in the Bemisia tabaci (Hemiptera: Aleyrodidae) species complex
Keywords:
Bemisia tabaci, mtCOI-I, mtCOI-II, ITS1, phylogenyAbstract
The analysis of sequence variation in the mitochondrial and ribosomal regions has been shown to provide an efficient method for the identification of species in a wide range of animal taxa. In order to assess its effectiveness in the discrimination of genetic groups of Bemisia tabaci (Gennadius) species complex, populations from ten cotton cultivars were analyzed. MtCOI, and ITS1 sequences were evaluated to find out the genetic diversity through phylogenetic methods, viz., maximum likelihood and network neighbor-net analysis. Analysis of mtCOI-1 or barcoding region did not reveal significant variation. On the contrary, the mtCOI-II region revealed the presence of three B. tabaci genetic groups or subgroups corresponded with the global data. However, the ITS1 region could not discriminate these groups of the species complex. Our results indicate that more than the barcoding and ITS1 regions, mtCOI-II region or 3′ end of the gene will be more appropriate for the identification of variations among the populations of B. tabaci species complex.
Se ha demostrado que el análisis de la variación de secuencias de las regiones mitocondriales y ribosomales provee un método eficiente para la identificación de especies en una amplia gama de taxa de animales. Para evaluar su eficacia en la discriminación de los grupos genéticos de las especies en el complejo de Bemisia tabaci (Gennadius), se analizó la población de diez cultivares de algodón. Se evaluaron los MtCOI y las secuencias ITS1 para averiguar la diversidad genética a través de métodos filogenéticos, como la máxima verosimilitud y el análisis del algoritmo de vecino de la red. El análisis de mtCOI-1 o la región de código de barra no reveló una variación significativa. Por el contrario, la región mtCOI-II reveló la presencia de tres grupos genéticos B. tabaci o subgrupos que correspondía con los datos globales. Sin embargo, la región ITS1 no podía discriminar estos grupos del complejo de especies. Nuestros resultados indican que más que el código de barras y las regiones ITS1, la región mtCOI-II o extremo 3′ del gen será más apropiada para la identificación de las variaciones entre las poblaciones del complejo de especies de B. tabaci.
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