Genetic evidence for diversity of spiralling whitefly, Aleurodicus dispersus (Hemiptera: Aleyrodidae) populations in India
Keywords:
Aleurodicus dispersus, populations, SSR primers, genetic diversity, dendrogramAbstract
The spiralling whitefly Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae), a highly polyphagous pest, has since about 2000 been an important pest of agricultural and horticultural crops in India. Despite its economic importance, little is known about the level and patterns of genetic variability in populations of the A. dispersus. In this study, we used the simple sequence repeats–polymerase chain reaction (SSR-PCR) technique was used to study the genetic diversity of A. dispersus in India. The present study is the first of its kind in India to utilize SSR markers for characterizing A. dispersus populations. A total of 356 different bands were scored using 18 primers for amplification. About 66.0% of alleles were polymorphic in A. dispersus populations. The SSR survey clearly detected moderate levels of polymorphism among the whitefly populations; multiple alleles were identified in many markers. The polymorphism information content (PIC) for SSR primers ranged from 0.0000 to 0.9541. Average genetic distances were estimated in our effort to investigate the level of DNA variation among the 7 geographic populations of A. dispersus from southern and northeastern India. The highest average genetic distance (0. 8182) was found between populations from Karnataka and Andhra Pradesh and Meghalaya and Mizoram. An UPGMA dendrogram based on similarity coefficients was constructed for the 7 populations analyzed. The A. dispersus population from Maharashtra was separated from those in the other 6 states viz., Tamil Nadu, Kerala, Andhra Pradesh, Karnataka, Mizoram and Meghalaya.
La mosca blanca espiral, Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae), es una plaga altamente polífaga, la cual desde acerca del año 2000 ha sido una plaga importante de cultivos agrícolas y hortícolas en la India. A pesar de su importancia económica, poco se sabe sobre el nivel y el patrón de variabilidad genética en las poblaciones de A. dispersus. En este estudio, utilizamos la técnica de una secuencia simple que se repite–reacción en cadena de la polimerasa (SSR-PCR) para estudiar la diversidad genética de A. dispersus en la India. El presente estudio es el primero de su clase en la India que utiliza marcadores de SSR para caracterizar las poblaciones de A. dispersus. Se obtuvo un total de 356 bandas diferentes utilizando 18 iniciadores (primers) para la amplificación. Acerca del 66.0% de los alelos fueron polimórficos en poblaciones de A. dispersus. El sondeo de SSR claramente detectó niveles moderados de polimorfismo entre las poblaciones de mosca blanca; se identificaron múltiples alelos en muchos de los marcadores. El contenido de información de polimorfismo (CIP) para los iniciadores de SSR varía desde 0.0000 hasta 0.9541. Se estimaron el promedio de las distancias genéticas en nuestro esfuerzo para investigar el nivel de variación del ADN entre las 7 poblaciones geográficas de A. dispersus del sur y del noreste de la India. Se encontró el promedio de la distancia genética más alto (0.8182) entre las poblaciones de Karnataka y Andhra Pradesh y Meghalaya y Mizoram. Se construyó un dendrograma UPGMA basado en los coeficientes de similitud de las 7 poblaciones analizadas. La población de A. dispersus de Maharashtra fue separada de los otros 6 estados: Tamil Nadu, Kerala, Andhra Pradesh, Karnataka, Mizoram y Meghalaya.
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