Evaluating Spore Count and Sporophorous Vesicle Size in Ovavesicula popilliae (Microsporidia: Ovavesiculidae) in Adult Japanese Beetles (Coleoptera: Scarabaeidae)

Authors

  • B. M. Petty Department of Entomology, 319 Agriculture Building, University of Arkansas, Fayetteville, Arkansas 72701
  • A. D. Tripodi Department of Entomology, 319 Agriculture Building, University of Arkansas, Fayetteville, Arkansas 72701
  • A. L. Szalanski Department of Entomology, 319 Agriculture Building, University of Arkansas, Fayetteville, Arkansas 72701
  • D. T. Johnson Department of Entomology, 319 Agriculture Building, University of Arkansas, Fayetteville, Arkansas 72701
  • D. C. Steinkraus Department of Entomology, 319 Agriculture Building, University of Arkansas, Fayetteville, Arkansas 72701

Keywords:

amplicon, DNA, host life stage, pathogen strains, Popillia japonica

Abstract

Ovavesicula popilliae is a microsporidian pathogen used as a biological control agent of the Japanese beetle (Popillia japonica Newman), and reportedly produces 32 spores within a sporophorous vesicle measuring 20.0-21.0 μm × 15.0-15.5 μm. We determined the sporophorous vesicles to have a mean size of 13.3 μm × 9.3 μm and each with a mean of 24.7 spores. Varying number of spores may be due to failed divisions of sporonts, death of spores after formation, host effects or different pathogen strains.

 

Ovavesicula popilliae es una microsporidio patógeno utilizado como agente de control biológico del escarabajo japonés (Popillia japonica Newman), que según reportes produce 32 esporas dentro de vesículas con medidas de 20.0-21.0 μm × 15.0-15.5 μm. Determinamos que las vesículas tienen un tamaño de 13.3 μm × 9.3 μm y una media de 24.7 esporas. La variación en el número de esporas puede deberse a fallas en la división de los sporonts, muerte de las esporas después de su formación, efectos del hospedero, o diferencias en las cepas del patógeno.

 

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Published

2014-02-23

Issue

Vol. 96, No. 4 (December 2013)

Section

Scientific Notes

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