Direct DNA Amplification from Fall Armyworm (Lepidoptera: Noctuidae) Samples
The direct amplification by PCR of the DNA in tissue samples of eggs and neonate larvae of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) was accomplished, and it is a new alternative for DNA amplification from fall armyworm samples. This method is simple, fast, economic, and accelerates studies on this polyphagous pest.
Se aplicó la técnica de PCR para amplificar el DNA directamente a partir de huevos y de larvas neonatas de Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae). Esta es una nueva alternativa para la amplificación del ADN de muestras del gusano cogollero. El método propuesto es simple, rápido, económico y agiliza los procedimientos para diversos estudios sobre esta plaga polífaga.
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