Direct DNA Amplification from Fall Armyworm (Lepidoptera: Noctuidae) Samples

Authors

  • Flavia Loto PROIMI-CONICET, Morphogenesis and Fermentation Laboratory, Avenida Belgrano y Pje. Caseros, T4001 MVB, San Miguel de Tucumán, Tucumán
  • Cintia M. Romero PROIMI-CONICET, Morphogenesis and Fermentation Laboratory, Avenida Belgrano y Pje. Caseros, T4001 MVB, San Miguel de Tucumán, Tucumán Facultad de Bioquímica, Química y Farmacia, Batalla de Ayacucho 471, T4000INI, Tucumán
  • Mario D. Baigorí PROIMI-CONICET, Morphogenesis and Fermentation Laboratory, Avenida Belgrano y Pje. Caseros, T4001 MVB, San Miguel de Tucumán, Tucumán Facultad de Bioquímica, Química y Farmacia, Batalla de Ayacucho 471, T4000INI, Tucumán
  • Licia M. Pera PROIMI-CONICET, Morphogenesis and Fermentation Laboratory, Avenida Belgrano y Pje. Caseros, T4001 MVB, San Miguel de Tucumán, Tucumán

Keywords:

DNA extraction, DNA purification, PCR amplification, sequencing, strain identification

Abstract

The direct amplification by PCR of the DNA in tissue samples of eggs and neonate larvae of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) was accomplished, and it is a new alternative for DNA amplification from fall armyworm samples. This method is simple, fast, economic, and accelerates studies on this polyphagous pest.

 

Se aplicó la técnica de PCR para amplificar el DNA directamente a partir de huevos y de larvas neonatas de Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae). Esta es una nueva alternativa para la amplificación del ADN de muestras del gusano cogollero. El método propuesto es simple, rápido, económico y agiliza los procedimientos para diversos estudios sobre esta plaga polífaga.

 

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Published

2014-02-23

Issue

Vol. 96, No. 4 (December 2013)

Section

Scientific Notes

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