Feeding Chamber for Myzus persicae Culture (Hemiptera: Aphididae)
Keywords:
aphid, survive, reproduceAbstract
Some feeding systems have been previously developed for aphids with excellent results, nevertheless, their costs are often high and they are difficult to construct. We developed a feeding chamber for Myzus persicae that is simple, rapidly assembled, and inexpensive. The system consisted of 2 plastic tumblers, one of which was covered with stretched Parafilm and the other with a piece of thin fabric, the diet consisted of 5% yeast extract and 30% sucrose in distilled water. We observed offspring and exuviae during the bioassay, furthermore, the mortality was below 10% at 72 hours. The results obtained with this feeding system indicated that aphids were able to survive and reproduce. This feeding chamber should prove useful in studies to test the effects of growth factors, nutrients, hormones, and chemical compounds on aphids, and it also might be used for other sucking insects.
Previamente se han desarrollado algunos sistemas de alimentación para áfidos con excelentes resultados, sin embargo, los costos son elevados y su elaboración es complicada. Nosotros desarrollamos una cámara de alimentación para Myzus persicae, la cual es simple, económica y fácil de ensamblar. El sistema consiste de dos vasos de plástico, uno de ellos cubierto con parafilm y el otro con organza, la dieta consiste de extracto de levadura al 5% y sacarosa al 30% disueltos en agua destilada. Durante los bioensayos se observó la presencia de exuvias y de ninfas de primer estadio de desarrollo, además a las 72 horas la mortalidad fue menor al 10%. Los resultados obtenidos con esta técnica, indican que los áfidos son capaces de sobrevivir y reproducirse. Este sistema de alimentación puede ser una herramienta muy importante en estudios donde se evalúen los efectos de factores de crecimiento, nutrientes, hormonas y compuestos químicos en áfidos, además, también podría utilizarse con otros insectos chupadores.
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