Resistance to Onion Thrips (Thysanoptera: Thripidae) in Onion Cultivars does not Prevent Infection by Iris Yellow Spot Virus Following Vector-Mediated Transmission
Abstract
Onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae), is a global pest of onion, Allium cepa L., and the principal vector of Iris yellow spot virus (IYSV) that can cause 100% crop losses. The purpose of this study was to evaluate onion cultivars resistant to T. tabaci feeding damage for their reaction to IYSV following exposure to viruliferous T. tabaci in both laboratory and field experiments. In the laboratory experiment, virus-free onion cultivars grown in pots were infested with 32 T. tabaci second instars collected from onions in an IYSV-infected field. In the complementary field experiment, virus-free onion plants in pots were moved to an onion field where IYSV was present. In both laboratory and field trials, plants were tested for IYSV by DAS-ELISA after 2 and 3 wk, respectively. Although plants were exposed to T. tabaci for a short period, IYSV was detected in all onion cultivars with the percentage of infected plants varying from 3 to 25% and 37 to 70% in the laboratory and field experiments, respectively. IYSV infection levels did not differ statistically between thrips-susceptible and thrips-resistant onion cultivars in laboratory and field experiments.El trips de la cebolla, Thrips tabaci Lindeman (Thysanoptera: Thripidae), es una plaga global de la cebolla, Allium cepa L., y el principal vector de Iris yellow spot virus (IYSV) que puede causar 100% de pérdidas del cultivo. El objetivo de este trabajo fue evaluar genotipos (resistentes al daño ocasionado por T. tabaci) por su reacción a IYSV transmitido por T. tabaci en condiciones de campo y laboratorio. En el experimento en laboratorio, plantas libres del virus fueron infestadas con 32 larvas de segundo instar de T. tabaci colectadas de un campo de cebolla infectado con IYSV. En el experimento complementario en campo, plantas libres de IYSV fueron llevadas al campo donde IYSV estaba presente. En ambos experimentos, laboratorio y campo, las plantas fueron examinadas con la prueba de DASELISA para detectar IYSV después de 2 y 3 semanas, respectivamente. Aunque las plantas fueron expuestas a T. tabaci por un periodo corto de tiempo, IYSV fue detectado en todos los genotipos con porcentajes de plantas infectadas que osciló entre 3 a 25% y 37 a 70% en los experimentos en el laboratorio y en el campo, respectivamente. Los niveles de infección de IYSV no fueron estadísticamente diferentes entre los genotipos susceptibles y resistentes a T. tabaci en los experimentos de campo y de laboratorio.
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