Effects of Thermal Stress on Lipid Peroxidation and Antioxidant Enzyme Activities of Oriental Fruit Fly, <em>Bactrocera dorsalis</em> (Diptera: Tephritidae)
Abstract
A change in temperature may be responsible for a variety of physiological stress responses in organisms, which are often associated with enhanced generation of reactive oxygen species (ROS) leading to oxidative damage. In the present study, the lipid peroxidation (LPO) levels of the oriental fruit fly, Bactrocera dorsalis (Hendel), were measured under thermal stress conditions of the relatively low (-5, -2.5, 0, and 5 °C) or high (35, 37.5, and 40 °C) temperatures. Subsequently, the time-related effect of thermal stress on activity of antioxidant enzymes including catalase (CAT), glutathione-S-transferases (GSTs), peroxidase (POX), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) were systematically determined. The results showed that LPO levels increased significantly in a time-dependent manner under thermal stress. The activities of CAT, GSTs, and SOD were significantly enhanced and likely provided a defense mechanism against oxidative damage due to the accumulation of ROS. POX and T-AOC levels were not significantly modified. These results suggest that thermal stress induces oxidative stress, and antioxidant enzymes likely play an important role in reducing oxidative damage in B. dorsalis.Un cambio en la temperatura puede ser responsable de una variedad de respuestas de estrés fisiológico en organismos, que son usualmente asociados al exceso en la generación de especies reactivas de oxígeno (ROS) llevando a un daño oxidativo. En el presente estudio, los niveles de peroxidación de lípidos (LPO) de la mosca de la fruta oriental, Bactrocera dorsalis (Hendel), fueron medidos bajo condiciones de estrés térmico con temperaturas relativamente bajas (-5, -2.5, 0, y 5 °C) o altas (35, 37.5, y 40 °C). Subsecuentemente, el efecto relacionado con el tiempo del estrés térmico sobre la actividad de enzimas antioxidantes incluyendo catalasa (CAT), glutathione-S-transferasa (GSTs), peroxidasa (POX), superóxido dismutasa (SOD), y capacidad antioxidante total (T-AOC) fueron determinadas sistemáticamente. Los resultados mostraron que los niveles de LPO aumentaron significativamente dependiendo del tiempo bajo estrés térmico. Las actividades de CAT, GSTs, y SOD aumentaron significativamente y posiblemente proporcionaron un mecanismo de defensa contra el daño oxidativo causado por la acumulación de ROS. Los niveles de POX y T-AOC no fueron modificados significativamente. Estos resultados sugieren que el estrés térmico induce estrés oxidativo, y que enzimas antioxidantes posiblemente juegan un papel importante reduciendo el daño oxidativo en B. dorsalis.
View this article in BioOne
Downloads
Published
Issue
Section
License
Copyright for any article published in Florida Entomologist is held by the author(s) of the article. Florida Entomologist is an open access journal. Florida Entomologist follows terms of the Creative Commons, Attribution Non-Commercial License (cc by-nc). By submitting and publishing articles in Florida Entomologist, authors grant the FOJ and Florida Entomologist's host institutions permission to make the article available through Internet posting and electronic dissemination, and to otherwise archive the information contained both electronically and in a hard printed version. When used, information and images obtained from articles must be referenced and cited appropriately. Articles may be reproduced for personal, educational, or archival purposes, or any non-commercial use. Permission should be sought from the author(s) for multiple, non-commercial reproduction. Written permission from the author(s) is required for any commercial reproduction.
