IDENTIFICATION OF FOUR COMMON Culex (Culex) (DIPTERA: CULICIDAE) SPECIES FROM FLORIDA WITH ISOENZYME ANALYSIS
Abstract
Females of four common Culex (Culex) species from Florida were analyzed for isoenzymes using polyacryamide gel electrophoresis. Ten enzymes that yielded 11 putative loci were studied. Most of the loci showed diagnostic characteristics in the four species, but four of the loci (glycerol-3-phosphate dehydrogenase [Gpd-2], hexokinase [Hk], isocitrate dehydrogenase [Idh-1], and malate dehydrogenase [Mdh]) could be used in sequence to identify the four Culex species. Culex salinarius and Cx. p. quinquefasciatus could be separated from Cx. restuans andCx. nigripalpus by Mdh locus. Culex salinarius could be distinguished from Cx. p. quinquefasciatus by Hk locus and Cx. nigripalpus could be distinguished from Cx. restuans, by Idh-1 and/or Gpd-2 loci. Randomly combined specimens of these four Culex species were identified accurately by using these enzyme loci.View this article in BioOne
Downloads
Published
Issue
Section
License
Copyright for any article published in Florida Entomologist is held by the author(s) of the article. Florida Entomologist is an open access journal. Florida Entomologist follows terms of the Creative Commons, Attribution Non-Commercial License (cc by-nc). By submitting and publishing articles in Florida Entomologist, authors grant the FOJ and Florida Entomologist's host institutions permission to make the article available through Internet posting and electronic dissemination, and to otherwise archive the information contained both electronically and in a hard printed version. When used, information and images obtained from articles must be referenced and cited appropriately. Articles may be reproduced for personal, educational, or archival purposes, or any non-commercial use. Permission should be sought from the author(s) for multiple, non-commercial reproduction. Written permission from the author(s) is required for any commercial reproduction.