Symposium: New Technologies for the Taxonomic Identification of Arthropods: Cytotaxonomy as a Tool for Identification Siblings of the Anopheles Quadrimaculatus Complex

  • Paul E. Kaiser


Wild populations of Anopheles quadrimaculatus were collected in the southeastern United States for cytogenetic studies. Two approaches, i.e., polytene chromosome comparisons and hybridization studies were used. Observations on the ovarian nurse cell polytene chromosomes indicated two distinct X chromosomes, one with a standard banding arrangement and the other with a fixed inversion. Three polymorphic inversions on the right arm of chromosome 3 were associated with the normal X (cytotype A). Individuals with the inverted X contained a polymorphic and a fixed inversion on both 3R and the left arm of chromosome 2 (cytotype B). The two types shared a small inversion on 2L and a complex arrangement on the left arm of chromosome 3 that included two distinct homokarotypes. The ovarian polytene chromosomes of the F"1 hybrids (A x B and reciprocal) demonstrated complete asynapsis in the X chromosome and 3L, and extensive asynapsis in the other arms. Differential mortality was expressed in F
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