Keywords
food safety
food defense
How to Cite
Abstract
Biofilms pose significant challenges in various industries due to their resistance to cleaning and sanitation. This publication explores the formation and implications of biofilms in food and agriculture settings. Biofilms play critical roles in microbial survival and have been linked to foodborne illness outbreaks, resulting in significant economic burdens. Factors contributing to biofilm formation include environmental conditions, material surfaces, agricultural practices, and processing conditions. Measures to prevent and control biofilms and their persistence in food processing environments include standard cleaning and sanitation procedures, as well as more innovative technologies. Understanding and addressing biofilm challenges are critical for ensuring food safety and minimizing economic losses throughout the food industry.
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References
Agüeria, D. A., C. Libonatti, and D. Civit. 2021. “Cleaning and Disinfection Programmes in Food Establishments: A Literature Review on Verification Procedures.” Journal of Applied Microbiology 131 (1): 23–35. https://doi.org/10.1111/jam.14962
Cámara, M., W. Green, C. E. MacPhee, et al. 2022. “Economic Significance of Biofilms: A Multidisciplinary and Cross-Sectoral Challenge.” Biofilms and Microbiomes 8: 42. https://doi.org/10.1038/s41522-022-00306-y
Cha, M.-Y., and J.-W. Ha. 2022. “Low-energy X-ray irradiation effectively inactivates major foodborne pathogen biofilms on various food contact surfaces.” Food Microbiology 106: 104054. https://doi.org/10.1016/j.fm.2022.104054
Chamberland, J., T. Messier, E. Dugat-Bony, et al. 2019. “Influence of Feed Temperature to Biofouling of Ultrafiltration Membrane During Skim Milk Processing.” International Dairy Journal 93: 99–105. https://doi.org/10.1016/j.idairyj.2019.02.005
Chitlapilly Dass, S., J. M. Bosilevac, M. Weinroth, et al. 2020. “Impact of Mixed Biofilm Formation with Environmental Microorganisms on E. coli O157:H7 Survival Against Sanitization.” npj Science of Food 4: 16. https://doi.org/10.1038/s41538-020-00076-x
Cornell College of Agriculture Science. n.d. “Produce Safety Rule.” Produce Safety Alliance. Accessed March 12, 2024. https://cals.cornell.edu/produce-safety-alliance/food-safety-modernization-act/produce-safety-rule
Di Martino, P. 2018. “Extracellular Polymeric Substances, a Key Element in Understanding Biofilm Phenotype.” AIMS Microbiology 4 (2): 274–288. https://doi.org/10.3934/microbiol.2018.2.274
Dutra, T. V., M. da Silva Fernandes, M. R. F. G. Perdoncini, M. N. dos Anjos, and B. A. de Abreu Filho. 2018. “Capacity of Escherichia coli and Staphylococcus aureus to Produce Biofilm on Stainless Steel Surfaces in the Presence of Food Residues.” Journal of Food Processing and Preservation 42 (4): e13574. https://doi.org/10.1111/jfpp.13574
Dzieciol, M., E. Schornsteiner, M. Muhterem-Uyar, B. Stessl, M. Wagner, and S. Schmitz-Esser. 2016. “Bacterial Diversity of Floor Drain Biofilms and Drain Waters in a Listeria monocytogenes Contaminated Food Processing Environment.” International Journal of Food Microbiology 223: 33–40. https://doi.org/10.1016/j.ijfoodmicro.2016.02.004
Fagerlund, A., E. Heir, T. Møretrø, and S. Langsrud. 2020. “Listeria monocytogenes Biofilm Removal Using Different Commercial Cleaning Agents.” Molecules 25 (4): 792. https://doi.org/10.3390/molecules25040792
Flemming, H.-C. 2016. “EPS—Then and Now.” Microorganisms 4 (4): 41. https://doi.org/10.3390/microorganisms4040041
Fonseca, J. M., S. D. Fallon, C. A. Sanchez, and K. D. Nolte. 2011. “Escherichia coli Survival in Lettuce Fields Following Its Introduction Through Different Irrigation Systems.” Journal of Applied Microbiology 110 (4): 893–902. https://doi.org/10.1111/j.1365-2672.2011.04942.x
Fusco, V., D. Chieffi, F. Fanelli, et al. 2020. “Microbial Quality and Safety of Milk and Milk Products in the 21st Century.” Comprehensive Reviews in Food Science and Food Safety 19 (4): 2013–2049. https://doi.org/10.1111/1541-4337.12568
Goff, H. D., and R. W. Hartel. 2013. “Cleaning and Sanitizing for Microbiological Quality and Safety.” In Ice Cream. Springer. https://doi.org/10.1007/978-1-4614-6096-1_13
Govaert, M., C. Smet, M. Baka, T. Janssens, and J. V. Impe. 2018. “Influence of Incubation Conditions on the Formation of Model Biofilms by Listeria monocytogenes and Salmonella Typhimurium on Abiotic Surfaces.” Journal of Applied Microbiology 125 (6): 1890–1900.
Gu, T., Y. Luo, Z. Jia, et al. 2024. “Surface topography and chemistry of food contact substances, and microbial nutrition affect pathogen persistence and symbiosis in cocktail Listeria monocytogenes biofilms.” Food Control 161: 110391. https://doi.org/10.1016/j.foodcont.2024.110391
Gu, T., A. Meesrisom, Y. Luo, et al. 2021. “Listeria monocytogenes Biofilm Formation as Affected by Stainless Steel Surface Topography and Coating Composition.” Food Control 130: 108275. https://doi.org/10.1016/j.foodcont.2021.108275
Guo, N., X. Bai, Y. Shen, and T. Zhang. 2023. “Target-Based Screening for Natural Products Against Staphylococcus aureus Biofilms.” Critical Reviews in Food Science and Nutrition 63 (14): 2216–2230. https://doi.org/10.1080/10408398.2021.1972280
Highmore, C. J., G. Melaugh, R. J. Morris, et al. 2022. “Translational Challenges and Opportunities in Biofilm Science: A BRIEF for the Future.” npj Biofilms and Microbiomes 8: 68. https://doi.org/10.1038/s41522-022-00327-7
Jang, H.-J., Y.-J. Choi, and J.-O. Ka. 2011. “Effects of Diverse Water Pipe Materials on Bacterial Communities and Water Quality in the Annular Reactor.” Journal of Microbiology and Biotechnology 21 (2): 115–123. https://doi.org/10.4014/jmb.1010.10012
Klančnik, A., K. Šimunović, M. Sterniša, D. Ramić, S. Smole Možina, and F. Bucar. 2021. “Anti-Adhesion Activity of Phytochemicals to Prevent Campylobacter jejuni Biofilm Formation on Abiotic Surfaces.” Phytochemistry Review 20 (1): 55–84. https://doi.org/10.1007/s11101-020-09669-6
Kwon, M., M. S. Hussain, and D. H. Oh. 2017. “Biofilm Formation of Bacillus cereus Under Food-Processing-Related Conditions.” Food Science and Biotechnology 26: 1103–1111. https://doi.org/10.1007/s10068-017-0129-8
Kyere, E. O., G. Foong, J. Palmer, J. J. Wargent, G. C. Fletcher, and S. Flint. 2020. “Biofilm Formation of Listeria monocytogenes in Hydroponic and Soil Grown Lettuce Leaf Extracts on Stainless Steel Coupons.” LWT 126: 109114. https://doi.org/10.1016/j.lwt.2020.109114
Lee, B. H., S. Cole, S. Badel-Berchoux, et al. 2019. “Biofilm Formation of Listeria monocytogenes Strains Under Food Processing Environments and Pan-Genome-Wide Association Study.” Frontiers in Microbiology 10: 2698. https://doi.org/10.3389/fmicb.2019.02698
Lucero-Mejía, J. E., S. de Jesús Romero-Gómez, and M. Hernández-Iturriaga. 2020. “A New Classification Criterion for the Biofilm Formation Index: A Study of the Biofilm Dynamics of Pathogenic Vibrio Species Isolated from Seafood and Food Contact Surfaces.” Journal of Food Science 85 (8): 2491–2497. https://doi.org/10.1111/1750-3841.15325
Miller, M. B., and B. L. Bassler. 2001. “Quorum Sensing in Bacteria.” Annual Review of Microbiology 55: 165–199. https://doi.org/10.1146/annurev.micro.55.1.165
Miser, D. L. 2011. Establishment Inspection Report (EIR) for the Environmental Assessment (EA) Conducted at Jensen Farms (FEI: 300844204). Department of Health and Human Services.
National Institutes of Health (NIH). 2002. “Research on Microbial Biofilms.” PA-03-047. https://grants.nih.gov/grants/guide/pa-files/PA-03-047.html
National Institutes of Health (NIH). 2007. “Immunology of Biofilms (R01).” PA-06-537. https://grants.nih.gov/grants/guide/pa-files/PA-06-537.html
Preda, V. G., and O. Săndulescu. 2019. “Communication is the key: Biofilms, Quorum Sensing, Formation and Prevention.” Discoveries Journals 7 (3): e10. https://doi.org/10.15190/d.2019.13
Redding, M., J. Zheng, J. Mowery, et al. 2024. “Microscopic and Transcriptomic Characterization of Listeria monocytogenes Aggregation and Biofilm Formation in Cantaloupe Juice.” Food Control 158: 110243. https://doi.org/10.1016/j.foodcont.2023.110243
Rossi, C., F. Maggio, C. Chaves‐López, et al. 2022. “Effectiveness of Selected Essential Oils and One Hydrolate to Prevent and Remove Listeria monocytogenes Biofilms on Polystyrene and Stainless Steel Food‐Contact Surfaces.” Journal of Applied Microbiology 132 (3): 1866–1876. https://doi.org/10.1111/jam.15376
Roy, P. K., M. G. Song, and S. Y. Park. 2022. “Impact of Quercetin Against Salmonella Typhimurium Biofilm Formation on Food–Contact Surfaces and Molecular Mechanism Pattern.” Foods 11 (7): 977. https://doi.org/10.3390/foods11070977
Saá Ibusquiza, P., M. Nierop Groot, A. Debán-Valles, T. Abee, and H. M. W. den Besten. 2015. “Impact of Growth Conditions and Role of sigB on Listeria monocytogenes Fitness in Single and Mixed Biofilms Cultured with Lactobacillus plantarum.” Food Research International 71: 140–145. https://doi.org/10.1016/j.foodres.2015.03.001
Sauer, K., P. Stoodley, D. M. Goeres, et al. 2022. “The Biofilm Life Cycle: Expanding the Conceptual Model of Biofilm Formation.” Nature Reviews Microbiology 20: 608–620. https://doi.org/10.1038/s41579-022-00767-0
Scallan, E., R. M. Hoekstra, F. J. Angulo, et al. 2011. “Foodborne Illness Acquired in the United States—Major Pathogens.” Emerging Infectious Diseases 17 (1): 7. https://doi.org/10.3201/eid1701.P11101
Schmidt, R. H. 2012. “Basic Elements of Equipment Cleaning and Sanitizing in Food Processing and Handling Operations.” EDIS 2012 (1). https://doi.org/10.32473/edis-fs077-2012
Sharan, M., D. Vijay, P. Dhaka, J. S. Bedi, and J. P. S. Gill. 2022. “Biofilms as a Microbial Hazard in the Food Industry: A Scoping Review.” Journal of Applied Microbiology 133 (4): 2210–2234. https://doi.org/10.1111/jam.15766
Simões, L. C., A. F. A. Chaves, M. Simões, and N. Lima. 2023. “Interactions Between Penicillium brevicompactum/Penicillium expansum and Acinetobacter calcoaceticus Isolated from Drinking Water in Biofilm Development and Control.” International Journal of Food Microbiology 384: 109980. https://doi.org/10.1016/j.ijfoodmicro.2022.109980
Song, P., Y. Xiao, Z. J. Ren, et al. 2021. “Electrochemical Biofilm Control by Reconstructing Microbial Community in Agricultural Water Distribution Systems.” Journal of Hazardous Materials 403: 123616. https://doi.org/10.1016/j.jhazmat.2020.123616
U.S. Centers for Disease Control and Prevention (CDC). 2018. “2011 Outbreak of Listeria Infections Linked to Whole Cantaloupes from Jensen Farms, Colorado.” Archived May 16, 2024. https://archive.cdc.gov/www_cdc_gov/listeria/outbreaks/cantaloupes-jensen-farms/index.html
U.S. Centers for Disease Control and Prevention (CDC). 2021. “Foodborne Disease Outbreak 2011 Case Definition.” National Notifiable Diseases Surveillance System (NNDSS). Last Reviewed April 16, 2021. https://ndc.services.cdc.gov/case-definitions/foodborne-disease-outbreak-2011/
U.S. Department of Agriculture, Food Safety and Inspection Service (USDA-FSIS). 2025. “Review of the Boar’s Head Listeria monocytogenes Outbreak—January 2025.” Last updated January 10, 2025. https://www.fsis.usda.gov/news-events/publications/review-boars-head-listeria-monocytogenes-outbreak-january-2025
U.S. Food and Drug Administration (FDA). 2022. “Outbreaks of Foodborne Illness.” Last updated September 26, 2022. https://www.fda.gov/food/recalls-outbreaks-emergencies/outbreaks-foodborne-illness
U.S. Food and Drug Administration (FDA). 2023. “Environmental Sampling.” Last updated June 13, 2023. https://www.fda.gov/food/sampling-protect-food-supply/environmental-sampling
U.S. Food and Drug Administration (FDA). 2024a. “FSMA Final Rule on Pre-Harvest Agricultural Water.” Last updated September 30, 2025. https://www.fda.gov/food/food-safety-modernization-act-fsma/fsma-final-rule-pre-harvest-agricultural-water
U.S. Food and Drug Administration (FDA). 2024b. “FSMA Final Rule on Produce Safety.” Last updated November 13, 2024. https://www.fda.gov/food/food-safety-modernization-act-fsma/fsma-final-rule-produce-safety
U.S. Food and Drug Administration (FDA). 2024c. “FSMA Rules & Guidance for Industry.” Updated January 30, 2024. Archived May 1, 2024. https://web.archive.org/web/20240501141516/https://www.fda.gov/food/food-safety-modernization-act-fsma/fsma-rules-guidance-industry
U.S. Food and Drug Administration (FDA). 2025. “FSMA Final Rule for Preventive Controls for Human Food.” Last updated January 6, 2025. https://www.fda.gov/food/food-safety-modernization-act-fsma/fsma-final-rule-preventive-controls-human-food
Wang, T., X. Dai, T. Zhang, C. Xin, Z. Guo, and J. Wang. 2022. “Formation and Microbial Composition of Biofilms in Drip Irrigation System Under Three Reclaimed Water Conditions.” Water 14 (8): 1216. https://doi.org/10.3390/w14081216
Wang, Y., L. Sun, L. Hu, Z. Wang, X. Wang, and Q. Dong. 2022. “Adhesion and Kinetics of Biofilm Formation and Related Gene Expression of Listeria monocytogenes in Response to Nutritional Stress.” Food Research International 156: 111143. https://doi.org/10.1016/j.foodres.2022.111143
Zhao, L., C. N. Poh, J. Wu, X. Zhao, Y. He, and H. Yang. 2022. “Effects of Electrolysed Water Combined with Ultrasound on Inactivation Kinetics and Metabolite Profiles of Escherichia coli Biofilms on Food Contact Surface.” Innovative Food Science & Emerging Technologies 76: 102917. https://doi.org/10.1016/j.ifset.2022.102917
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