Effect of Rosmarinic Acid on Cancer Promoting E.coli (NC101)

Abstract

Natural products, such as Rosmarinic acid (RA), can be utilized as alternatives to therapies or treatments that may have harmful consequences to the host. RA has demonstrated anti-cancer properties and antibacterial activity. The objective of this research was to evaluate the potential of RA to reduce growth of a Colorectal cancer (CRC)-promoting bacteria, such as E coli-NC101, which produces a genotoxin that promotes DNA damage in intestinal epithelial cells (IECs). NC101 was cultured with a series of 2-fold dilutions of RA ranging from 10mg/mL to 0.01mg/mL, and OD600 was measured to determine the minimum inhibitory concentration (MIC). RA significantly decreased E.coli-NC101 stationary growth at 5mg/mL (p<0.001). Growth curve analysis showed that this RA-induced decrease delayed the growth of NC101 at this concentration. Further experiments will determine the effect of RA on NC101-induced DNA damage.